CRISPR/Cas9 APOBEC3H APOBEC3CH - Method for generation of APOBEC3H and APOBEC3CH double-knocked out cat using CRISPR/Cas9 system

• Main Authors: KONG, IL KEUN, LEE, KYEONG LIM, LEE, SANG RYEUL
• Format: Patent
• Language: English; Korean
• Published: 06.03.2018
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention relates to a method for simply and efficiently producing a transgenic cat in which APOBEC3H and APOBEC3CH genes are knocked out using a CRISPR / Cas9 system. The present invention further relates to a use of the transgenic cat thus produced thereby. The cat model produced by the method of the present invention useful in investigation of HIV-1 infection and vaccine. 본 발명은 CRISPR/Cas9 시스템을 이용하여 APOBEC3H 및 APOBEC3CH 유전자가 넉아웃된 형질전환 고양이를 간편하고 효율적으로 제조하는 방법 및 이에 따라 제조된 형질전환 고양이의 용도에 관한 것이다. 본 발명의 방법에 따라 생산된 고양이 모델은 HIV-1 감염 및 벡신 연구에 유용하게 활용될 수 있다.