Method for gene editing in microalgae using RGEN RNP

• Main Authors: BAEK, KWANG RYUL, BAE, SANG SU, JIN, EON SEON, KIM, DUK HYOUNG
• Format: Patent
• Language: English; Korean
• Published: 01.11.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are a method for editing gene of microorganism without introduction of DNA using a composite of RNA-guided engineered nuclease (RGNE) ribonucleoprotein (RNP), and a gene-edited mutant microorganism. According to the present invention, the gene editing method makes it possible to accurately...