Method for gene editing in microalgae using RGEN RNP

Provided are a method for editing gene of microorganism without introduction of DNA using a composite of RNA-guided engineered nuclease (RGNE) ribonucleoprotein (RNP), and a gene-edited mutant microorganism. According to the present invention, the gene editing method makes it possible to accurately...

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Bibliographic Details
Main Authors BAEK, KWANG RYUL, BAE, SANG SU, JIN, EON SEON, KIM, DUK HYOUNG
Format Patent
LanguageEnglish
Korean
Published 01.11.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided are a method for editing gene of microorganism without introduction of DNA using a composite of RNA-guided engineered nuclease (RGNE) ribonucleoprotein (RNP), and a gene-edited mutant microorganism. According to the present invention, the gene editing method makes it possible to accurately and selectively edit desired gene without introduction of DNA. 본 발명은 RGNE (RNA-guided engineered nuclease) RNP의 복합체를 이용하여 DNA 도입 없이 미세조류의 유전자를 교정하는 방법, 및 유전자가 교정된 미세조류 변이체를 제공한다. 본 발명에 따른 유전자 교정 방법은 DNA 도입 없이 원하는 유전자를 정확하게 선택적으로 교정할 수 있다는 이점을 갖는다.
Bibliography:Application Number: KR20170041761