THE SEQUENCE-SPECIFIC CELLULAR UPTAKE OF SPHERICAL NUCLEIC ACID NANOPARTICLE CONJUGATES

• Main Authors: CHOI CHUNG HANG J, MIRKIN CHAD A, NARAYAN SUGUNA P, HAO LIANGLIANG, AUYEUNG EVELYN
• Format: Patent
• Language: English; Korean
• Published: 07.07.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

조밀하게 팩킹되고 고도로 배향되어 나노입자 표면에 부착된 폴리뉴클레오티드 가닥들로 이루어진 구형 핵산(SNA)은 통상의 핵산 전달시 난관을 극복할 수 있다. 본 개시내용은 G가 증량된 SNA가, 다른 뉴클레오티드가 증량된 SNA에 비하여 수 배 더 큰 세포로의 흡수를 보임을 입증한다. 본 개시내용은, 다른 나노입자 계에도 적용 가능하므로, 치료제의 나노입자 기반 세포 내 전달에 중요한 설계 고려사항을 확립해 주는, SNA의 세포 내 전달을 최대화하기 위한 유효 전략을 제공한다. Spherical nucleic acids (SNAs), consisting of densely packed, highly oriented polynucleotide strands attached to the surface of nanoparticles, are able to overcome the typical challenges of nucleic acid delivery. The present disclosure demonstrates that G-rich SNAs exhibit several-fold higher uptake into cells relative to SNAs rich in other nucleotides. This disclosure provides an effective strategy to maximize the intracellular delivery of SNAs, which is applicable to other nanoparticle systems, thus establishing an important design consideration for nanoparticle-based intracellular delivery of therapeutics.