RNA METHOD FOR NANOPORE RNA CHARACTERISATION

• Main Authors: JAYASINGHE LAKMAL, WHITE JAMES, HERON ANDREW JOHN, GARALDE DANIEL RYAN, TURNER DANIEL JOHN
• Format: Patent
• Language: English; Korean
• Published: 27.06.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

본 발명은 표적 RNA 폴리뉴클레오티드가 막횡단 세공에 대하여 이동함에 따라 하나 이상의 측정치를 취함으로써 표적 RNA 폴리뉴클레오티드의 특징을 규명하는 새로운 방법에 관한 것이다. 상기 이동은 DNA 헬리카제 효소에 의해 제어된다. 본 발명은 또한, RNA 폴리뉴클레오티드를, 이와 결합하는 DNA 헬리카제를 증가시키도록 변형시킨 변형된 RNA 구축물에 관한 것이다. The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.