MMETHOD OF DISTINGUISHING MESENCHYMAL STEM CELLS

• Main Authors: CHANG CHIA CHI, LI YUAN TSUNG, SHEN SHIH PEI, LIU WEI TING, LIN YUN, HSUAN CHANG YO
• Format: Patent
• Language: English; Korean
• Published: 23.06.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

본원에서는 태반-관련된 조직으로부터 유래된 세포의 일차 배양물에서 중간엽 줄기세포(MSC)를 구별하는 방법이 제공된다. 또한, 본원에서는 태반-관련된 조직으로부터 유래된 세포의 일차 배양물에서 MSC 집단의 순도를 증가시키는 방법이 제공된다. 추가로, 본원에서는 염증성 환경에서 더 반응성인 MSC 집단을 단리시키는 방법이 제공된다. 상기 방법들은 각각 EphA2의 마커에 의해서 세포를 분류하는 단계를 포함한다. A method of distinguishing mesenchymal stem cells (MSCs) in a primary culture of cells derived from a placenta-related tissue is provided. Also provided is a method of increasing the purity of MSC population in a primary culture of cells derived from a placenta-related tissue. Further provided is a method of isolating a MSC population which is more responsive in an inflammatory environment. Said methods each comprise a step of sorting the cells by a marker of EphA2.