A novel -Mannosidase and producing method thereof

• Main Authors: PARK, YANG SOON, JEON, HO JIN, JIN, HYUN KYUNG, CHOI, DAE KEON, YOON, KI HONG
• Format: Patent
• Language: English; Korean
• Published: 15.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to: beta-mannosidase, which is a novel enzyme; a hexane molecule for coding the same; a recombinant vector comprising the hexane molecule; a recombinant microorganism having the hexane molecule or the recombinant vector inserted in a host cell; a composition comprising the beta-mannosidase; and a method for manufacturing the beta-mannosidase. The method is capable of degrading saccharides having D-mannose as a main ingredient using the beta-mannosidase. According to the method, the D-mannose can be manufactured. In addition, the method can be used in an industrial manner by adding the beta-mannosidase to a biomass and converting the biomass to the saccharides. 본 발명은 신규한 효소인 베타-만노시다제, 이를 코딩하는 핵산 분자, 상기 핵산 분자를 포함하는 재조합 벡터, 상기 핵산 분자 또는 상기 재조합 벡터가 숙주세포에 삽입되어 있는 재조합 미생물, 상기 베타-만노시다제를 포함하는 조성물, 상기 베타-만노시다제를 제조하는 방법에 관한 것으로, 상기 베타-만노시다제를 이용하면 D-만노스를 주성분으로 하는 당류를 분해할 수 있으며, 이러한 방법에 따라 D-만노스를 제조할 수 있고, 또한 바이오매스에 상기 베타-만노시다제를 첨가하여 바이오매스를 당으로 전환시키는 등으로 산업적으로 활용할 수 있다.