Novel macrolactam glycoside derivatives chemoenzymatic method for preparation thereof and antibacterial composition comprising the same

• Main Authors: KANG, HAN YOUNG, YOON, YEO JOON, LEE, DONG GUN, SHINDE PRAMOD B
• Format: Patent
• Language: English; Korean
• Published: 07.10.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

The present invention relates to a novel macrolactam glycoside derivative, a method for chemoenzymatically producing the same, and an antibacterial composition including the same as an active ingredient. According to a method for chemoenzymatically producing novel macrolactam glycoside derivative of the present invention, a non-natural macrolactam glycoside derivative is produced by glycosylating chemically synthesized macrolactam provided from the outside by using a microorganism for internally producing a macrolactam glycoside derivative and an enzyme in charge of the biosynthesis of the macrolactam glycoside derivative. Compared to natural macrolactam glycoside, the macrolactam glycoside derivative produced according to the present invention has an equivalent or higher antibacterial activity and shows improved metabolic stability, so the macrolactam glycoside derivative can be useful in discovering a novel structure antibacterial material. 본 발명은 신규 마크로락탐 글리코시드 유도체, 이의 화학효소적 제조방법 및 이를 유효성분으로 포함하는 항균용 조성물에 관한 것이다. 본 발명의 신규한 마크로락탐 글리코시드 유도체를 제조하는 화학효소적 방법은 내재적으로 마크로락톤 글리코시드 유도체를 생산하는 미생물 및 상기 마크로락톤 글리코시드 유도체의 생합성에 관여하는 효소를 이용하여 외부로부터 제공되는 화학적으로 합성된 마크로락탐을 글리코실화함으로써 비천연 마크로락탐 글리코시드 유도체를 제조하는 방법으로서, 본 발명의 방법에 의해 제조된 마크로락탐 글리코시드 유도체는 천연 마크로락톤 글리코시드와 비교하여 동등 이상의 항균활성을 가지며 나아가 개선된 대사 안정성을 나타내므로 신규한 구조의 항균 물질 발굴에 유용하게 사용할 수 있다.