ALKENE PRODUCTION

Provided in the present invention is a microorganism cell, which can produce at least one type or terminal end alkene from at least one type of short-chain fatty acids, and is genetically modified to comprise the followings, wherein the short-chain fatty acids are C4-C10 fatty acids. At least one fi...

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Main Authors BUELTER THOMAS, GILCH STEFAN, FABER KURT, HALL MELANIE, DENNIG ALEXANDER, HAAS THOMAS, THIESSENHUSEN ANJA
Format Patent
LanguageEnglish
Korean
Published 05.09.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided in the present invention is a microorganism cell, which can produce at least one type or terminal end alkene from at least one type of short-chain fatty acids, and is genetically modified to comprise the followings, wherein the short-chain fatty acids are C4-C10 fatty acids. At least one first generic mutant increases the expression of enzyme (E1) selected from CYP152 peroxygenase system in comparison with a wild-type cell. At least one second genetic mutant increases the expression of at least one type of NAD(P)+ oxidoreductase (E2) and the corresponding medium protein in comparison with the wild-type cell. 본 발명은 1종 이상의 단쇄 지방산으로부터 1종 이상의 말단 알켄을 생성시킬 수 있으며, 하기를 포함하도록 유전적으로 변형되어 있고, 여기서 상기 단쇄 지방산은 C4-C10 지방산인 미생물 세포를 제공한다: - 야생형 세포에 비해 CYP152 퍼옥시게나제 계열에서 선택되는 효소 (E)의 발현을 증가시키는 하나 이상의 제1 유전자 돌연변이, 및 - 야생형 세포에 비해 1종 이상의 NAD(P)+ 옥시도리덕타제 (E) 및 상응하는 매개체 단백질의 발현을 증가시키는 하나 이상의 제2 유전자 돌연변이.
Bibliography:Application Number: KR20160022425