STREPTOMYCES HYGROSCOPICUS MUTANT STRAIN WITH INCREASED PRODUCTIVITY OF RAPAMYCIN

• Main Authors: SUH, JUNG WOO, YOON, DONG HOON, KIM, BYOUNG KOOK, MOON, SUNG HYUN, YU, SEONG SEON
• Format: Patent
• Language: English; Korean
• Published: 23.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are: a Streptomyces hygroscopicus CKDBR 0807 strain which produces rapamycin, which is an immune inhibitor, at high concentration; and a method for producing rapamycin using the same. The strain of the present invention is able to produce the immune inhibitor, rapamycin at high concentration, thereby being very economical. The present invention also provides information about base sequence variation of genes which may affect the improvement of rapamycin productivity and is obtained by comparing draft genome sequencing results of the strain of the present invention to a wild-type strain. Accordingly, the present invention can be usefully used for industrial mass-production of the immune inhibitor, rapamycin. 본 발명은 면역억제제 라파마이신을 고농도로 생산하는 스트렙토마이세스 하이그로스코피쿠스 CKDBR 0807 균주 및 이를 이용하여 라파마이신을 생산하는 방법을 제공한다. 본 발명의 균주는 면역억제제 라파마이신을 고농도로 생산할 수 있기 때문에 매우 경제적이다. 본 발명의 균주와 야생형 균주의 드래프트 지놈 시퀀싱 결과를 비교하여 수득한 라파마이신 생산성 향상에 영향을 미칠 수 있는 유전자의 염기서열 변이에 대한 정보를 제공한다. 따라서, 본 발명은 면역억제제 라파마이신의 산업적인 대량 생산을 위하여 유용하게 사용될 수 있다.