METHOD FOR RAPIDLY AND QUANTATIVELY ANALYZING BETA-LACTAM ANTIBIOTIC RESISTANCE AND METHOD FOR EVALUATING RESISTANCE USING THE SAME

• Main Authors: KIM, SEON HWA, KANG, JEONG WOO, PARK, HAE CHUL, JANG, YANG HO, SO, BYUNG JAE, LEE, KWANG JICK
• Format: Patent
• Language: English; Korean
• Published: 03.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a method for analyzing beta-lactam antibiotic resistance comprising: (a) a pretreatment step including a step of culturing a strain resistant to beta-lactam antibiotics and a strain susceptible to the antibiotics, a step of exposing the cultured strains to the beta-lactam antibiotics, and a step of filtering the resultant product; and (b) measuring resistance against the beta-lactam antibiotics of the pretreated strains by using LC-MS/MS in which two mass spectrometers (MS) are connected to a liquid chromatography (LC). According to the present invention, the method for analyzing beta-lactam antibiotic resistance can quantify antibiotic resistance, and measure the resistance in 2 hours using the simple pretreatment method. 본 발명은 (a) 베타-락탐계 항생제에 내성인 균주 및 상기 항생제에 감수성인 균주를 배양한 후, 상기 배양된 균주를 베타-락탐계 항생제에 노출시킨 다음, 여과하는 단계를 포함하는 전처리 단계, 및 (b) 액체크로마토그래피(LC)에 두 개의 매스 스펙트로미터(MS)가 연결되어 되어 있는 LC-MS/MS를 이용하여 상기 전처리된 균주의 베타-락탐계 항생제에 대한 내성을 측정하는 단계를 포함하는 베타-락탐계 항생제 내성의 분석방법에 관한 것이다.