ENHANCED PRODUCTION OF A RECOMBINANT POLYPEPTIDE BY OVEREXPRESSION OF MPC1

• Main Authors: PARK, EUN YOUNG, KIM, KYUNG EUN, SUH, HYE YOUNG, SUNG, HEE KYUNG, KIM, SUN KYU
• Format: Patent
• Language: English; Korean
• Published: 18.07.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are a recombinant cell in which overexpression of a mitochondrial pyruvate carrier (MPC) gene is induced; a composition used to produce a target polypeptide, which includes the recombinant cell and/or a recombinant vector including an MPC gene; and a production method of a target polypeptide using the recombinant cell. The recombinant cell can be practically used to massively produce various therapeutic proteins. The production method of a target polypeptide comprises the following step: cultivating a recombinant cell in which overexpression of an MPC gene is induced. 미토콘드리아 피루베이트 수송체 (mitochondrial pyruvate carrier; MPC) 유전자의 과발현이 유도된 재조합 세포, 상기 재조합 세포 및/또는 MPC 유전자를 포함하는 재조합 벡터를 포함하는 목적 폴리펩타이드 생산용 조성물, 및 상기 재조합 세포를 이용하여 목적 폴리펩타이드의 생산 방법이 제공된다.