SYNTHESIS OF SLOWLY DIGESTIBLE STARCH BY MALTOSE-BINDING-PROTEIN FUSED 4,6-ALPHA-GLUCANOTRANSFERASE AND THE USES THEREOF

• Main Authors: PARK, JONG TAE, YOO, MIN JEE, JUNG, DA HAN, KIM, JUNG EUN
• Format: Patent
• Language: English; Korean
• Published: 08.07.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention provides a method for producing slowly digestible starch, and a food produced by using slowly digestible starch produced through the method. The method comprises the steps of: producing a recombinant vector including a maltose-binding-protein coding gene and a gene for coding a 4,6αGT, 4,6-α-glucanotransferase derived from Lactobacillus reuteri; transforming the produced recombinant vector to a colon bacillus; separating and refining maltose-binding-protein fused 4,6αGT, 4,6-α-glucanotransferase from the transformed colon bacillus; and reacting the refined maltose-binding-protein fused 4,6-α-glucanotransferase with starch. 본 발명은 말토오스 결합 단백질 코딩 유전자와 락토바실러스 루테리()에서 유래된 4,6-α-글루카노트랜스퍼라제(4,6αGT, 4,6-α-glucanotransferase) 단백질을 코딩하는 유전자를 포함하는 재조합 벡터를 제조하는 단계; 상기 제조된 재조합 벡터를 대장균에 형질전환시키는 단계; 상기 형질전환된 대장균으로부터 말토오스 결합 단백질 융합 4,6-α-글루카노트랜스퍼라제 효소를 분리 및 정제하는 단계; 및 상기 정제된 말토오스 결합 단백질 융합 4,6-α-글루카노트랜스퍼라제 효소와 전분을 반응하는 단계를 포함하는 것을 특징으로 하는 지소화성 전분의 제조방법 및 상기 방법에 의해 제조된 지소화성 전분을 이용하여 제조된 식품을 제공한다.