SYNTHESIS OF SLOWLY DIGESTIBLE STARCH BY MALTOSE-BINDING-PROTEIN FUSED 4,6-ALPHA-GLUCANOTRANSFERASE AND THE USES THEREOF
The present invention provides a method for producing slowly digestible starch, and a food produced by using slowly digestible starch produced through the method. The method comprises the steps of: producing a recombinant vector including a maltose-binding-protein coding gene and a gene for coding a...
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Main Authors | , , , |
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Format | Patent |
Language | English Korean |
Published |
08.07.2016
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention provides a method for producing slowly digestible starch, and a food produced by using slowly digestible starch produced through the method. The method comprises the steps of: producing a recombinant vector including a maltose-binding-protein coding gene and a gene for coding a 4,6αGT, 4,6-α-glucanotransferase derived from Lactobacillus reuteri; transforming the produced recombinant vector to a colon bacillus; separating and refining maltose-binding-protein fused 4,6αGT, 4,6-α-glucanotransferase from the transformed colon bacillus; and reacting the refined maltose-binding-protein fused 4,6-α-glucanotransferase with starch.
본 발명은 말토오스 결합 단백질 코딩 유전자와 락토바실러스 루테리()에서 유래된 4,6-α-글루카노트랜스퍼라제(4,6αGT, 4,6-α-glucanotransferase) 단백질을 코딩하는 유전자를 포함하는 재조합 벡터를 제조하는 단계; 상기 제조된 재조합 벡터를 대장균에 형질전환시키는 단계; 상기 형질전환된 대장균으로부터 말토오스 결합 단백질 융합 4,6-α-글루카노트랜스퍼라제 효소를 분리 및 정제하는 단계; 및 상기 정제된 말토오스 결합 단백질 융합 4,6-α-글루카노트랜스퍼라제 효소와 전분을 반응하는 단계를 포함하는 것을 특징으로 하는 지소화성 전분의 제조방법 및 상기 방법에 의해 제조된 지소화성 전분을 이용하여 제조된 식품을 제공한다. |
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Bibliography: | Application Number: KR20140192995 |