PHARMACEUTICAL COMPOSITION FOR PARKINSON'S DISEASE CONTAINING CATALASE FUSION PROTEIN

• Main Authors: PARK, JIN SEO, KWON, HYEOK YIL, CHO, YOON SHIN, KIM, DUK SOO, UM, WON SIK, CHO, SUNG WOO, KWON, OH SHIN, LEE, KEUN WOOK, HAN, KYU HYUNG, CHOI, SU YOUNG, KIM, DAE WON
• Format: Patent
• Language: English; Korean
• Published: 06.01.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Parkinson′s disease is a neurodegenerative disease that dopamine neurons are gradually removed from the substantia nigra of the brain. According to the present invention, it has been clarified that a PEP-1-catalase fusion protein can protect dopamine neurons from an oxidation stress in an SH-SY5Y neuroblastoma cell and a Parkinson′s disease animal model. The PEP-1-catalase fusion protein has been confirmed to move well into an SH-SY5Y cell and the substantia nigra of the brain through a western blot analysis. The PEP-1-catalase fusion protein remarkably inhibits generation of active oxygen species induced by MPP^+ and DNA fragmentation, and induces survival of the SH-SY5Y cell as a result. Moreover, immunohistochemical analysis data using a TH antibody and cresyl violet coloration represents that the PEP-1-catalase fusion protein remarkably protects a dopamine cell in the substantia nigra with respect to the oxidation stress such as MPTP. Accordingly, the PEP-1-catalase fusion protein may be used for preventing and treating Parkinson′s disease. 파킨슨병은 뇌의 흑색질 내에서 도파민 뉴런이 점차 상실되는 신경퇴행성 질환이다. 본 발명자들은 PEP-1-카탈라제 융합단백질이 SH-SY5Y 신경아세포종 세포 및 파킨슨병 동물모델에서 산화 스트레스로부터 도파민 뉴런을 보호할 수 있음을 밝혀내었다. PEP-1-카탈라제 융합단백질은 SH-SY5Y 세포 내로 그리고 뇌의 흑색질 내로 잘 이동함을 웨스턴 블랏 분석으로 확인하였다. PEP-1-카탈라제 융합단백질은 MPP로 유도되는 활성산소종 생성 및 DNA 단편화를 현저히 저해하였으며, 결과적으로 SH-SY5Y 세포의 생존을 유도했다. 나아가, TH 항체 및 크레실 바이올렛 염색을 이용한 면역조직화학 분석 데이타는 PEP-1-카탈라제 융합단백질이 MPTP와 같은 산화 스트레스에 대하여 흑색질 내의 도파민 세포를 현저히 보호함을 나타낸다. 따라서, PEP-1-카탈라제 융합단백질은 파킨슨병의 예방 및 치료 용도로 이용 가능하다.