USE OF THERMOPHILIC NUCLEASES FOR DEGRADING NUCLEIC ACIDS

• Main Authors: MCGRATH JESSICA, TRUEHEART JOSHUA
• Format: Patent
• Language: English; Korean
• Published: 24.11.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 발명은 핵산을 생체내 및/또는 원위치에서 분해시키기 위한 호열성 뉴클레아제의 용도에 관한 것이고, 상기 호열성 뉴클레아제는 숙주 세포에 이종이고 외부적으로 첨가되기 보다는 상기 숙주 세포에 의해 생산된다. 본 발명은 또한 상기 방법에 따라 생산된 유전자 변형된 세포에 관한 것이다. 본 발명은 바이오매스 또는 바이오매스-유래된 산물에서 재조합 DNA의 생물 활성을 불활성화시키는데 특히 이롭다. The present invention relates to the use of a thermophilic nuclease for degrading nucleic acids in vivo and/or in situ, wherein the thermophilic nuclease is heterologous to the host cell and is produced by the host rather than being added exogenously. The present invention further relates to a genetically modified cell which was produced according to the above method. The present invention is particularly beneficial in inactivating the biological activity of recombinant DNA in biomass or biomass-derived products.