OPTICAL BASED GRAPHENE BIOSENSOR WITH APTAMERS, A PREPARATION METHOD OF THE SAME AND A METHOD FOR DETECTING A TARGET MATERIAL USING THE SAME

• Main Authors: KIM, MI GYEONG, OH, YU KYOUNG, LEE, JAI WOO, SHON, YU NA
• Format: Patent
• Language: English; Korean
• Published: 11.03.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to an optical based graphene biosensor using aptamers, a preparation method thereof, and a method for detecting a target material using the same. More specifically, according to the present invention, when a fluorescent aptamer forms a double helix structure with a complementary nucleic acid sequence thereof, and is fixated to a graphene substrate by a fixing material so that is exposed to the target material, the fluorescent material marked on the aptamer is reacted with the surface of graphene to lose the fluorescence while the fluorescent aptamer forms a single helix structure composite with the target material by the stronger attraction force than the complementary nucleic acid sequence thereof, thereby the target material can be quantitatively detected.