SYSTEM FOR IN VITRO DETECTION AND/OR QUANTIFICATION BY FLUOROMETRY

• Main Authors: GALDIERO MASSIMO, ROSSI VERONICA LUCIA, FRANCINI FRANCO, JAFRANCESCO DAVID, FERORELLI GIUSEPPE
• Format: Patent
• Language: English; Korean
• Published: 03.06.2014
• Subjects: CALCULATING; COMPUTING; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a system for the in vitro detection and/or quantification, by fluorometry, of at least one analyte in a sample of fluid constituting a biological material, in particular for an immunological test, including a radiation source, followed by an optical splitter for splitting the main beam into a sample-energizing beam and a reference beam, with a first photodetector means for detecting a fluorescence ray emitted by the sample and a second photodetector means for the reference beam, said system also including a generator outputting a sinusoidal carrier signal and at least one digital demodulation signal, and a digital processing means for processing, by demodulation, the signals from the two photodetector means in order to extract a fluorescence value that is characteristic of the amplitude of the fluorescence ray and a second reference value that is characteristic of the amplitude of the reference beam.