DETECTING METHOD FOR KPCS AND MBLS USING BROTH MICRODILUTION METHOD WITH ??-LACTAMASE INHIBITORS

• Main Authors: JEONG, SEOK HOON, SONG, WON KEUN
• Format: Patent
• Language: English; Korean
• Published: 12.03.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a liquid culture medium microdilution method to detect the production of Klebsiella pneumoniae cabapenemases (KPCs) and metalo-beta-lactamases (MBLs) from clinically separated gram-negative bacillus using various beta-lactamase inhibitors. The cabapenemase detection method is made of a Mueller-Hinton liquid culture medium which includes a diluted solution obtained by preforming a serial doubling dilution on imipenem or meropenem, and which includes or does not include aminophenyl-boronic (APB) acid, phenyl-boronic (PB) acid, cloxacillin (CLX), dipicolinic acid (DPA), or EDTA. The present invention proves that the liquid culture medium microdilution method, which uses imipenem, includes or does not include phenyl-boronic acid, cloxacillin, and EDTA, provides the most effective result of KPC and MBL detection.