SIMPLE AND RAPID PRE-TEST SYSTEM FOR EXPRESSION AND CYTOTOXICITY OF THE HUMAN GENES IN E. COLI
The present invention relates to a technique which expresses heterologous protein, desirably human protein from E. coli which does not include T7 RNA polymerase under control of a T7 promoter. More specifically, the present invention relates to a use of increasing expression of heterologous protein...
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Main Authors | , , |
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Format | Patent |
Language | English Korean |
Published |
06.02.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to a technique which expresses heterologous protein, desirably human protein from E. coli which does not include T7 RNA polymerase under control of a T7 promoter. More specifically, the present invention relates to a use of increasing expression of heterologous protein from E.coli which does not include T7 RNA polymerase using a vector including the T7 promoter and N-terminal HA tag sequence, to E.coli which includes the vector, and in which the expression of heterologous protein is increased without T7 RNA polymerase, to a method for expressing or preparing the heterologous protein using the E.coli, and to a method for analyzing functions of the heterologous protein. In addition, the present invention relates to a method for simply and rapidly diagnosing intracelluar expression and cytotoxicity of human genes using the vector. |
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Bibliography: | Application Number: KR20120082719 |