A USE OF MAMMALIAN VECTOR INCLUDING T7 PROMOTER AND N-TERMINAL HA TAG FOR THE OVER-EXPRESSION OF HUMAN GENES IN E. COLI

The present invention relates to a technique which expresses heterologous protein, desirably human protein from E. coli which does not include T7 RNA polymerase under control of a T7 promoter. More specifically, the present invention relates to a use of increasing expression of heterologous protein...

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Bibliographic Details
Main Authors KIM, GOO YOUNG, RHIM, HYANG SHUK, MOON, JEONG MI
Format Patent
LanguageEnglish
Korean
Published 06.02.2014
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a technique which expresses heterologous protein, desirably human protein from E. coli which does not include T7 RNA polymerase under control of a T7 promoter. More specifically, the present invention relates to a use of increasing expression of heterologous protein from E.coli which does not include T7 RNA polymerase using a vector including the T7 promoter and N-terminal HA tag sequence, to E.coli which includes the vector, and in which the expression of heterologous protein is increased without T7 RNA polymerase, to a method for expressing or preparing the heterologous protein using the E.coli, and to a method for analyzing functions of the heterologous protein. In addition, the present invention relates to a method for simply and rapidly diagnosing expression and toxicity inside cells of human genes using the vector.
Bibliography:Application Number: KR20120082718