MICRO CULTURE DISH, AND METHOD FOR CELL CULTURE USING THE SAME

• Main Authors: KIM, MIN CHEOL, CHUN, JONG SIK, LEE, JUNG HOON, LEE, SUNG JUN
• Format: Patent
• Language: English; Korean
• Published: 22.01.2014
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a micro cell culture apparatus for effectively culturing a single cell, and a method for culturing a micro cell by desirably using the same. The micro cell culture apparatus according to the present invention is characterized by comprising: a culture chamber array substrate wherein multiple culture chambers are arranged, on the surface thereof, in a home form having 0.5-5 mm in diameter and 0.1-1 mm in depth; a porous thin film sealing the culture chamber by covering the surface of the culture chamber array substrate as a thin film having an opening of 0.1-1 μm. The method for culturing a micro cell according to the present invention is characterized by comprising: a first step for preparing a sample suspension by suspending a collected sample into a buffer solution; a second step for filling the inside of the culture chamber of the culture chamber array substrate with the sample suspension; a third step for sealing the culture chamber by covering the surface of the culture chamber array substrate with the porous film; a fourth step for culturing by immersing, into nutrient broth, the culture chamber array substrate covered with the porous film. [Reference numerals] (AA) Microorganism; (BB) Wipe; (CC) Supporting body; (DD) Nutrient broth