WASH SOLUTION AND METHOD FOR AFFINITY CHROMATOGRAPHY

The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell prot...

Full description

Saved in:
Bibliographic Details
Main Authors FRAUENSCHUH ACHIM, BILL KURT
Format Patent
LanguageEnglish
Korean
Published 08.10.2012
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Online AccessGet full text

Cover

Abstract The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell proteins, while also increasing product concentration in the eluate and maintaining a high percent yield of recovered product.
AbstractList The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell proteins, while also increasing product concentration in the eluate and maintaining a high percent yield of recovered product.
Author FRAUENSCHUH ACHIM
BILL KURT
Author_xml – fullname: FRAUENSCHUH ACHIM
– fullname: BILL KURT
BookMark eNrjYmDJy89L5WQwCXcM9lAI9vcJDfH091Nw9HNR8HUN8fB3UXDzD1JwdHPz9PMMiVRw9gjy93UM8XcPcgzwiORhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHeQUYGhkBkYGlqYOZoTJwqAHSyKhY
ContentType Patent
DBID EVB
DatabaseName esp@cenet
DatabaseTitleList
Database_xml – sequence: 1
  dbid: EVB
  name: esp@cenet
  url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP
  sourceTypes: Open Access Repository
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Chemistry
Sciences
ExternalDocumentID KR20120109506A
GroupedDBID EVB
ID FETCH-epo_espacenet_KR20120109506A3
IEDL.DBID EVB
IngestDate Fri Oct 25 05:41:08 EDT 2024
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Language English
Korean
LinkModel DirectLink
MergedId FETCHMERGED-epo_espacenet_KR20120109506A3
Notes Application Number: KR20127015524
OpenAccessLink https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121008&DB=EPODOC&CC=KR&NR=20120109506A
ParticipantIDs epo_espacenet_KR20120109506A
PublicationCentury 2000
PublicationDate 20121008
PublicationDateYYYYMMDD 2012-10-08
PublicationDate_xml – month: 10
  year: 2012
  text: 20121008
  day: 08
PublicationDecade 2010
PublicationYear 2012
RelatedCompanies NOVARTIS AG
RelatedCompanies_xml – name: NOVARTIS AG
Score 2.8276875
Snippet The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering...
SourceID epo
SourceType Open Access Repository
SubjectTerms CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Title WASH SOLUTION AND METHOD FOR AFFINITY CHROMATOGRAPHY
URI https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121008&DB=EPODOC&locale=&CC=KR&NR=20120109506A
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8NAEB5qFfWmValaZUHJLRjMs4cg2zxMrXmQptqeSl4FUZJiI_59Z2OqPfW2uwOzD5j9ZmZnZgFuc4StWBEVXs4kiZeyVOGTfqbyspYvUhERRsiZoeh6ijORnqbytAUf61yYuk7od10cESUqRXmv6vt6-e_EMuvYytVd8oZD5YMd6SbXWMesGpagceZAtwLf9A3OMPRRyHlhTWOvQLKg0B3YRUVaZfJgvQxYXspyE1TsI9gLkF9RHUPrvezAgbH-e60D-27z5I3NRvpWJyC90rFDxv7zhPmVCPVM4lqR45sETTlCbXvoDaMZMZzQd2nkP4Y0cGancGNbkeHwOP38b7fzUbi5VvEM2kVZ5F0gcSzcx1lfRGDJ0MxItVhNUmmBKhyqLbGcn0NvG6eL7eRLOGTd3_C2HrSrz6_8CvG2Sq7rY_oBGzl9pg
link.rule.ids 230,309,783,888,25576,76876
linkProvider European Patent Office
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1JS8NAFH7UKtabVsWl6oCSWzCYpe0hSJrFqWmSkqbankK2gihJsRH_vm9iqj31NsyDNwu8-d4-AHcZwlakiAovp5LES2mi8HE_7fJyL1skIiKMkDFD0XEVOpWeZ_KsAR_rWpiqT-h31RwRJSpBeS-r93r578QyqtzK1X38hlPFoxWoBldbx6wbltDjjIFqjj3D0zldV22fc_2KxqJAsqBoO7CLSnaXyYP5MmB1KctNULEOYW-M_PLyCBrvRRta-vrvtTbsO3XIG4e19K2OQXrVJpRMvNGU-ZWI5hrEMQPqGQRNOaJZ1tAdBnOiU99ztMB78rUxnZ_ArWUGOuVx-fDvtKHtb-5VPIVmXuTZGZAoEh6itC8isKRoZiS9qBsn0gJVOFRbIjk7h842ThfbyTfQooEzCkdD176EA0b6TXXrQLP8_MquEHvL-Lq6sh8rYICZ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=WASH+SOLUTION+AND+METHOD+FOR+AFFINITY+CHROMATOGRAPHY&rft.inventor=FRAUENSCHUH+ACHIM&rft.inventor=BILL+KURT&rft.date=2012-10-08&rft.externalDBID=A&rft.externalDocID=KR20120109506A