WASH SOLUTION AND METHOD FOR AFFINITY CHROMATOGRAPHY

The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell prot...

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Bibliographic Details
Main Authors FRAUENSCHUH ACHIM, BILL KURT
Format Patent
LanguageEnglish
Korean
Published 08.10.2012
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, and a nonbuffering salt, preferably at high pH, greater than 8.0, is effective in removing impurities, such as high molecular weight species and host cell proteins, while also increasing product concentration in the eluate and maintaining a high percent yield of recovered product.
Bibliography:Application Number: KR20127015524