Polynucleotide for preparing human G-CSF and use thereof

PURPOSE: A fusion polynucleotide having ER transition signal sequence and a base sequence encoding human-derived target recombinant proteins is provided to enhance the expression of the polynucleotide. CONSTITUTION: A fusion polynucleotide contains a base sequence encoding PPAE(prophenoloxidase acti...

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Bibliographic Details
Main Authors GOO, TAE WON, CHUNG, KYUNG TAE, PARK, JEONG HAE, KANG, SEOK WOO
Format Patent
LanguageEnglish
Korean
Published 05.06.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: A fusion polynucleotide having ER transition signal sequence and a base sequence encoding human-derived target recombinant proteins is provided to enhance the expression of the polynucleotide. CONSTITUTION: A fusion polynucleotide contains a base sequence encoding PPAE(prophenoloxidase activating enzyme), PDI(protein disulfide isomerase), or BX(bombyxin) ER transition signal sequence and human-derived target recombinant proteins. The human-derived target recombinant protein is human G-CSF. A method for preparing human G-CSF comprises: a step of introducing an expression vector to insect cells or insects; a step of culturing the cells or breeding the insects; and a step of isolating the human G-CSF from the cells or insects.
Bibliography:Application Number: KR20100119134