HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS BY A MULTIPLE TRANSFECTION PROCEDURE OF MAR SEQUENCES

A purified and isolated DNA sequence has protein production increasing activity. Said DNA sequence comprises at least one bent DNA element, and at least one binding site for a DNA binding protein. It is a MAR nucleotide with the sequence SEQ lD No 27, a sequence complementary thereof, or a sequence...

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Bibliographic Details
Main Authors MERMOD NICOLAS, CALABRESE DAVID, GIROD PIERRE ALAIN, PUTTINI STEFANIA, SAUGY DAMIEN, BUCHER PHILIPP, NGUYEN DUC QUANG
Format Patent
LanguageEnglish
Korean
Published 13.10.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A purified and isolated DNA sequence has protein production increasing activity. Said DNA sequence comprises at least one bent DNA element, and at least one binding site for a DNA binding protein. It is a MAR nucleotide with the sequence SEQ lD No 27, a sequence complementary thereof, or a sequence having at least 70% identity with said sequence. Advantageously the bent DNA element contains at least 10% of dinucleotide TA, and/or at least 12% of dinucleotide AT on a stretch of 100 contiguous base pairs.
Bibliography:Application Number: KR20117023131