RECOMBINANT SARS-COV NSP12 AND THE USE THEREOF, AND THE METHOD FOR PRODUCING IT

PURPOSE: A recombinant SARS-CoV nsp12 protein having SARS-CoV genome replication activity is provided to overexpress and easily purify in high purity. CONSTITUTION: A recombinant SARS-CoV nsp12 protein has amino acids having a number of histidines at N-terminal. In the recombinant SARS-CoV nsp12 pro...

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Bibliographic Details
Main Authors AHN, DAE GYUN, OH, JONG WON
Format Patent
LanguageEnglish
Korean
Published 09.02.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: A recombinant SARS-CoV nsp12 protein having SARS-CoV genome replication activity is provided to overexpress and easily purify in high purity. CONSTITUTION: A recombinant SARS-CoV nsp12 protein has amino acids having a number of histidines at N-terminal. In the recombinant SARS-CoV nsp12 protein, nine amino acid sequences are deleted at N-terminal. The recombinant SARS-CoV nsp12 protein is expressed by an expression vector pTrcSARSnsp12. The SARS-CoV nsp12 has an amino acid sequence of sequence number 1. A method for producing the recombinant SARS-CoV nsp12 protein comprises: a step of transforming a host cell with the expression vector; a step of culturing the host cells to inducing expression recombinant SARS-CoV nsp12; a step of crushing the host cells; and a step of isolating and purifying the protein from crushed cells.
Bibliography:Application Number: KR20107027765