FLUORESCENCE BASED ASSAY TO DETECT SODIUM/CALCIUM EXCHANGER (NCX)"REVERSE MODE" MODULATING COMPOUNDS

Transporters are an emerging target family with enormous potential, offering scientific and economic opportunities. The sodium/calcium exchanger is an important mechanism for removing Ca 2+ from diverse cells. In heart, it extrudes Ca 2+ that has entered through Ca 2+ channels to initiate contractio...

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Bibliographic Details
Main Authors FREY MANFRED, GEIBEL SVEN, LICHER THOMAS
Format Patent
LanguageEnglish
Korean
Published 30.12.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:Transporters are an emerging target family with enormous potential, offering scientific and economic opportunities. The sodium/calcium exchanger is an important mechanism for removing Ca 2+ from diverse cells. In heart, it extrudes Ca 2+ that has entered through Ca 2+ channels to initiate contraction, while Na + enters the heart cell. It is of considerable interest to identify compounds that modulate, not only the calcium export activity (forward mode), but also the calcium import activity (reverse mode) of sodium/calcium exchangers. The present invention is directed to a fluorescence-based assay for detecting NCX "reverse mode" modulating compounds. It further refers to a kit of parts comprising cells expriming NCX and the use of the kit of parts to test a compound for activity as an agonist or antagonist of NCX.
Bibliography:Application Number: KR20107020410