METHOD FOR THE REFOLDING OF PROTEIN CONTAINING DISULFIDE BOND USING IONIC LIQUIDS

PURPOSE: A method for refolding a protein with disulfide bond using ionic liquid is provided to optimize refolding process time and to improve refolding efficiency. CONSTITUTION: A method for refolding a protein performed by adding ionic liquid to a buffer solution containing Tris-HCl, EDTA, reduced...

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Bibliographic Details
Main Authors HA, SUNG HO, BAE, SANG WOO, KOO, YOON MO, CHANG, WOO JIN
Format Patent
LanguageEnglish
Korean
Published 14.10.2010
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:PURPOSE: A method for refolding a protein with disulfide bond using ionic liquid is provided to optimize refolding process time and to improve refolding efficiency. CONSTITUTION: A method for refolding a protein performed by adding ionic liquid to a buffer solution containing Tris-HCl, EDTA, reduced glutathione and oxidized glutathione. A cation part of the ionic liquid is an imidazolium of 1-4 carbon atoms of substituted alkyl group.
Bibliography:Application Number: KR20090029446