A METHOD FOR AN EFFEICIENT SELECTION OF CELL LINE PRODUCING RECOMBINANT PROTEINS

PURPOSE: A method for sorting overexpressing recombinant cell line is provided to select cell clone of high productivity using MTX of low concentration. CONSTITUTION: A method for sorting overexpressing recombinant cell line is performed using an expression vector. The expression vector comprises: a...

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Bibliographic Details
Main Authors KIM, JONG MOOK, SONG, YOO CHEOL, LEE, HYUN JOO, CHANG, MIN SEOK, CHO MYUNG SAM, KIM, MAN SU
Format Patent
LanguageEnglish
Korean
Published 08.10.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: A method for sorting overexpressing recombinant cell line is provided to select cell clone of high productivity using MTX of low concentration. CONSTITUTION: A method for sorting overexpressing recombinant cell line is performed using an expression vector. The expression vector comprises: a gene expression cassette having a selection marker gene which is unoperably linked with polyadehylation signal(polyA) and a gene expression cassette encoding a target recombinant protein which is operably linked with poly A. A method for sorting overexpressing recombinant cell line comprises: a step of cleaving an expression vector having a gene expression cassette encoding a target recombinant protein; and a step of linearizing the expressionvector.
Bibliography:Application Number: KR20100028222