THE ESTIMATING METHOD OF THE ELASTICITY OF DNA TARGETING DRUG-BOUND DNA AND THE ESTIMATING METHOD OF THE DEGREE OF BINDING TO DNA USING THE AFOREMENTIONED METHOD

• Main Authors: PARK, JIN SUNG, LEE, NAM KYUNG, HYUN, JU YONG, LEE, KYUNG J, HONG, SEOK CHEOL
• Format: Patent
• Language: English; Korean
• Published: 10.06.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

PURPOSE: A method for measuring elasticity of DNA targeting drug-bound DNA and a method for measuring binding degree of DNA and DNA targeting drug are provided to find physical property of DNA. CONSTITUTION: A method for measuring elasticity of DNA targeting drug-bound DNA is performed by measuring tension and length to a binding DNA. A method for measuring relation of tension and length is defined by WLC having changing continuous length by formula 3 in force-length curve of WLC model of formula 1 in case of lack of interelation between kinks generated by binding. The binding with DNA targeting drug is performed in a proper tension and salt concentration.