INTEGRATED NUCLEIC ACID ANALYIS

• Main Authors: LAM HEUNG CHUAN, ULRICH HANS THOMANN, TAN EUGENE, BOGDANOV VALERY LEONIDOVICH, KELLOGG GREGORY JOHN, WRIGHT JOHN A, SELDEN RICHARD F
• Format: Patent
• Language: English; Korean
• Published: 07.04.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics. 본 발명은 핵산 분석을 수행하기 위한 완전히 통합된 미세유체 시스템을 제공한다. 본 발명의 공정은 샘플 수집, 핵산 추출 및 정제, 증폭, 시퀀싱, 및 분리와 검출을 포함한다. 본 발명은 또한 광학 검출 시스템 및 생물학적 분자의 분리 및 검출 방법을 제공한다. 특히, 본 발명의 다양한 양상은 하나 또는 복수의 미세유체 챔버 또는 채널내부의, 복수의 생물학적 분자, 전형적으로 형광 염료-표지된 핵산의 동시 분리 및 검출을 가능케 할 수 있다. 핵산은 6가지 이상의 염료로 표지될 수 있고, 각각의 염료는 독특한 피크 방출 파장을 갖는다. 본 발명의 시스템 및 방법은 특히 DNA 단편 사이징 적용, 예컨대, 유전자 핑거프린팅 및 DNA 시퀀싱 적용, 예컨대, 임상 진단학에 유용하다.