PLANT TRANSFORMATION VECTOR AND MARKER FREE TRANSGENIC PLANTS USING STRESS INDUCIBLE SITE-SPECIFIC RECOMBINATION

PURPOSE: A method for preparing a transformant of a plant using a vector is provided to simply remove a selection marker and prepare the selection marker free transformant of plant through self-fertilization and cross-fertilization. CONSTITUTION: An expression vector for transforming plant which is...

Full description

Saved in:
Bibliographic Details
Main Authors LEE, SI MYUNG, LIM, SUN HYUNG, WOO, HEI JONG, CHO, HYUN SEUK, SHUN, GONG SIK
Format Patent
LanguageEnglish
Published 03.02.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:PURPOSE: A method for preparing a transformant of a plant using a vector is provided to simply remove a selection marker and prepare the selection marker free transformant of plant through self-fertilization and cross-fertilization. CONSTITUTION: An expression vector for transforming plant which is able to remove a selection marker contains stress-inducing peroxidase promoter, site-specific recombinase gene and recombinase recognition site. The recombinase gene is a stie specific recombinase FLP gene which is expressed from stress-inducing peroxidase promoter. The recognition site is a sequence of recombinase recognition frt. The expression vector is pWHF-P(deposit number: KACC 95088P). A method for preparing plant which is able to remove selection marker comprises: a step of cutting leaves of the plant; a step of infecting transformed Agrobacterium sp. microorganism; a step of inducing redifferentiation using a selection medium in which hydrogen peroxide is added; and a step of analyzing transformation of the gene.
Bibliography:Application Number: KR20080072282