METHOD OF PRODUCING CLOSTRIDIUM BOTULINUM TOXIN USING A MEDIA CONTAINING PLANT DERIVED COMPONENTS AND A FLEXIBLE CLOSED CONTAINER

• Main Authors: KIM, HACK WOO, HONG, HYUNG PYO, YANG, GI HYEOK, JUNG, HYUN HO, YOON, YOUNG SUK, LEE, BYUNG KOOK
• Format: Patent
• Language: English
• Published: 24.11.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: A method for producing Clostridium botulinum toxin using plant-derived component-containing medium is provided to extracellular express the Clostridium botulinum toxin without any installation for anaerobic condition. CONSTITUTION: A method for producing Clostridium botulinum toxin comprises: a step of culturing Clostridium botulinum toxin in a medium containing plant-derived component to express Clostridium botulinum toxin extracellularly; and a step of collecting a part containing the Clostridium botulinum toxin then separating toxin. The medium containing plant-derived component is a medium containing glucose, yeast extract and python peptone; a medium containing glucose, yeast extract, python peptone and plant tryptone; and a medium containing glucose, yeast extract, python peptone and soytone.