PRODUCTION OF THE ANTIGENIC EPITOPE RECOMBINANT PROTEIN OF HELICOBACTER PYLORI FLAA REACTIVE TO HUMAN ANTISERA AND ITS METHOD

An epitope recombinant protein which is reactive to human antisera and producing method thereof are provided to improve health care of stomach and duodenum by widely develop the diagnosis method. A method for manufacturing an epitope recombinant protein which is reactive to human antisera of FlaA of...

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Main Authors SEO, JI HYUNG, NIZAMUTDINOV DAMIR, BAIK, SEUNG CHUL, LEE, WOO KON, YOUN, HEE SHANG, KANG, HYUNG LYUN, CHO, MYUNG JE, RHEE, KWANG HO, OH, CHANG HWAN, JUN, JIN SOO, SONG, JAE YOUNG
Format Patent
LanguageEnglish
Korean
Published 28.07.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:An epitope recombinant protein which is reactive to human antisera and producing method thereof are provided to improve health care of stomach and duodenum by widely develop the diagnosis method. A method for manufacturing an epitope recombinant protein which is reactive to human antisera of FlaA of helicobater pylori comprise: a step of amplifying antigen gene and epitope partial gene fragment; a step of treating with a restriction enzyme; a step of isolating and purifying the cut gene; a step of treating with same restriction enzyme in a vector; a step of expressing protein and mapping; and a step of expressing a clone and obtaining the expressed protein.
Bibliography:Application Number: KR20080007094