NOVEL GENE ISOLATED FROM CORYNEBATERIUM, INOSINE-PRODUCING CORYNEBACTERIUM TRANSFORMED BY THE SAME GENE AND METHOD OF PRODUCING INOSINE USING THE SAME

A method for manufacturing an inosine using a novel gene isolated from a Corynebacterium sp. microorganism is provided to directly accumulate the inosine with high concentration and produce the inosine with high concentration and high yield. A gene derived from a Corynebacterium ammoniagenes has a s...

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Main Authors KIM, HYOUNG SEOK, SIM, JAE ICK, CHOI, HYE JIN, KIM, CHUL HA, KIM, JEONG HWAN, CHOI, JONG SOO, BAEK, MIN JI, KWON, JUNG GUN, KWON,RA, HWANG, SOO YOUN, AHN, TAE MIN
Format Patent
LanguageEnglish
Korean
Published 13.07.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for manufacturing an inosine using a novel gene isolated from a Corynebacterium sp. microorganism is provided to directly accumulate the inosine with high concentration and produce the inosine with high concentration and high yield. A gene derived from a Corynebacterium ammoniagenes has a sequence of the sequence number 1(SEQ ID NO:1). A recombinant vector is made by inserting the gene of the sequence number 1 into pHC131T vector. The transformed Corynebacterium sp. microorganism has inosine productivity which gene expression is improved. The Corynebacterium sp. microorganism is Corynebacterium ammoniagenes CN 04-0094 (KCCM-10906). The inosine is produced by culturing the Corynebacterium ammoniagenes CN 04-0094 (KCCM-10906) and isolating the inosine from medium.
Bibliography:Application Number: KR20080002661