PURIFING METHOD OF PORPHYRANS EXTRACTED FROM PORPHYRA YEZOENSIS USING COLUMN CHROMATOGRAPHY

Provided is a method for isolating and purifying porphyrans with a high yield, which avoids a need for the use of a harmful toxic solvent such as cetyl pyridinium chloride or ethanol. The method for isolating and purifying porphyrans comprises the steps of: (a) dipping dried laver (porphyra yezoensi...

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Bibliographic Details
Main Authors KWON, MI JIN, NAM, TAEK JEONG
Format Patent
LanguageEnglish
Published 11.01.2007
Subjects
CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
THEIR PREPARATION OR CHEMICAL WORKING-UP
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Summary:Provided is a method for isolating and purifying porphyrans with a high yield, which avoids a need for the use of a harmful toxic solvent such as cetyl pyridinium chloride or ethanol. The method for isolating and purifying porphyrans comprises the steps of: (a) dipping dried laver (porphyra yezoensis) in deionized water, heating it to perform hydrothermal extraction, filtering the extract, and adding ethanol to the filtrate to obtain crude porphyrans; (b) passing the crude porphyrans through a DEAE-52 column equilibrated with water, fractionating the product with 0.5M NaCl solution, performing dialysis with water, and concentrating the produce under vacuum; (c) removing lipids from the resultant powder by using chloroform and methanol, and passing the product through a Sephacryl S-200 column; and (d) carrying out gel filtration by using a Sephadex G-75 column packed with water.
Bibliography:Application Number: KR20050059808