DETECTION METHOD FOR SPECIFIC BIOMOLECULAR INTERACTIONS USING FRET BETWEEN METAL NANOPARTICLE AND QUANTUM DOT

• Main Authors: HONG, MI YOUNG, NAM, SUNG HUN, KIM, HAK SUNG, OH, EUN KEU, LEE, DO HOON
• Format: Patent
• Language: English
• Published: 22.08.2006
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MANUFACTURE OR TREATMENT THEREOF; MEASURING; NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS,MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES ASDISCRETE UNITS; NANOTECHNOLOGY; PERFORMING OPERATIONS; PHYSICS; TESTING; TRANSPORTING

The present invention relates to (1) a method for detecting a specific binding for bio-molecules; (2) a method for detecting a presence of target molecules within a specimen; and (3) a method for measuring a quantity of target molecules existing in a specimen, in which a pair of energy donor and energy acceptor displaying a FRET phenomenon is used to guide a specific binding between a pair of bio-molecules. According to the present invention, the process is conducted rapidly and easily without a label so as to screen a biochemical substance inhibiting a specific binding between a pair of bio-molecules in an ultra-high speed and measure a quantity of the substance and thereby, it can be applied to develop a novel drug. Further, this method can be used to analyze a characteristic such as change of the amount of carbohydrates in a glycoprotein derived from various cells as a drug candidate and thereby, is applicable for a quality control of proteins etc.