METHOD OF CONSTRUCTING HYBRIDOMA PRODUCING ANTIGEN-SPECIFIC ANTIBODY USING SINGLE ANTIGEN-SPECIFIC B LYMPHOCYTE AND METHOD OF PRODUCING MONOCLONAL ANTIBODY

It is intended to provide a method of constructing a hybridoma producing an antigen-specific antibody with the use of an antigen-specific B lymphocyte and a method of producing a monoclonal antibody. The method of constructing a hybridoma producing an antigen-specific antibody comprises selecting a...

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Main Authors MURAGUCHI ATSUSHI, SUZUKI MASAYASU, TOHBO KIHACHIRO, UENO MINORU, NAKAZATO HIROYOSHI, TAMIYA EIICHI, KISHI HIROYUKI
Format Patent
LanguageEnglish
Published 09.01.2006
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:It is intended to provide a method of constructing a hybridoma producing an antigen-specific antibody with the use of an antigen-specific B lymphocyte and a method of producing a monoclonal antibody. The method of constructing a hybridoma producing an antigen-specific antibody comprises selecting a B lymphocyte reacting specifically with a certain antigen (an antigen-specific B lymphocyte), culturing the thus selected antigen-specific B lymphocyte, and fusing the antigen-specific B lymphocytes having proliferated by the culture with myeloma cells to give hybridomas. Using a hybridoma constructed by this method, a monoclonal antibody is produced.
Bibliography:Application Number: KR20057018241