FIBRINOLYTIC ENZYME HAVING IMPROVED FIBRINOLYTIC ACTIVITY, ISOLATED FROM KOREAN EARTHWORM (EISENIA ANDREI), AND METHOD FOR PRODUCING THE SAME WITH IMPROVED CONVENIENCE AND REDUCED COST

PURPOSE: Fibrinolytic enzyme isolated from Korean earthworm (Eisenia andrei), a gene encoding the same enzyme, a recombinant expression vector containing the same gene, and E. coli transformed by the same vector for massive expression are provided, thereby cheaply and easily expressing and purifying...

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Bibliographic Details
Main Authors PARK, SOON CHEOL, LEE, MYUNG SIK, TAK, EUN SIK, JO, SUNG JIN
Format Patent
LanguageEnglish
Korean
Published 02.02.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: Fibrinolytic enzyme isolated from Korean earthworm (Eisenia andrei), a gene encoding the same enzyme, a recombinant expression vector containing the same gene, and E. coli transformed by the same vector for massive expression are provided, thereby cheaply and easily expressing and purifying a high purity thrombolytic protein having improved fibrinolytic activity. CONSTITUTION: The fibrinolytic enzyme isolated from Korean earthworm (Eisenia andrei) has the amino acid sequence set forth in SEQ ID NO:2. The gene encoding the fibrinolytic enzyme isolated from Korean earthworm (Eisenia andrei) has the nucleotide sequence set forth in SEQ ID NO:1. The recombinant expression vector pGEX/efe2 contains the gene of SEQ ID NO:1. The transformed E. coli BL21/efe2 is produced by transforming E. coli BL21 with the recombinant expression vector pGEX/efe2. A method for mass producing the fibrinolytic enzyme comprises culturing the transformed E. coli BL21/efe2 in an appropriate medium.
Bibliography:Application Number: KR20030051369