METHOD FOR SEPARATING (γ-HYMELEU4)-CYCLOSPORIN A FROM THE CULTURED BROTH OF SEBEKIA BENIHANA WITH IMPROVED SELECTIVITY

PURPOSE: A method for separating (γ-HyMeLeu4)-cyclosporin A from the cultured broth of Sebekia benihana is provided, thereby improving separation selectivity of (γ-HyMeLeu4)-cyclosporin A which is useful as hair restorer. CONSTITUTION: The method for separating (γ-HyMeLeu4)-cyclosporin A from the cu...

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Bibliographic Details
Main Authors HAN, KYU BOEM, RYU, HYO LIM
Format Patent
LanguageEnglish
Korean
Published 10.01.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: A method for separating (γ-HyMeLeu4)-cyclosporin A from the cultured broth of Sebekia benihana is provided, thereby improving separation selectivity of (γ-HyMeLeu4)-cyclosporin A which is useful as hair restorer. CONSTITUTION: The method for separating (γ-HyMeLeu4)-cyclosporin A from the cultured broth of Sebekia benihana comprises the steps of: culturing Sebekia benihana(KCTC 9173) in a medium containing 1% yeast extract, 0.5% soybean powder, 0.7% glucose, 1% soluble starch and 0.005% CaCO3 at 30 deg. C for 36 hours; adding 500 mg/l of cyclosporin A dissolved in DMSO(dimethyl sulfoxide) into the cultured medium of Sebekia benihana(KCTC 9173) to convert cyclosporin A into (γ-HyMeLeu4)-cyclosporin A; culturing the mixture at 30 deg. C for 36 hours; and adsorbing (γ-HyMeLeu4)-cyclosporin A from the cultured medium of Sebekia benihana(KCTC 9173) using a hydrophobic resin such as Amberlite XAD-16 or Amberlite XAD-1180.
Bibliography:Application Number: KR20040106192