HUMANIZED MONOCLONAL POLYPEPTIDE SPECIFIC FOR HUMAN 4-1BB MOLECULE AND PHARMACEUTICAL COMPOSITION COMPRISING THE SAME, IN WHICH THE HUMANIZED MONOCLONAL POLYPEPTIDE IS USED AS AUTOIMMUNE DISEASE TREATING DRUG OR IMMUNE-SUPPRESSIVE DRUG WITHOUT ADVERSE SIDE EFFECTS

• Main Authors: LEE, UNN HWA, KWON, BYOUNG SE, KWON, MYUNG HEE, KIM, JI YOUNG
• Format: Patent
• Language: English; Korean
• Published: 06.10.2004
• Edition: 7
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

PURPOSE: A humanized monoclonal polypeptide specific for human 4-1BB molecule and a pharmaceutical composition comprising the same are provided. The humanized monoclonal polypeptide has high affinity to human 4-1BB molecule and similar amino acid sequence to human antibody, and is thus useful as an autoimmune disease treating drug or immune-suppressive drug without adverse side effects. CONSTITUTION: The humanized monoclonal polypeptide specific for human 4-1BB molecule comprising the amino acid sequence set forth in SEQ ID NO:5 is produced from a cell line Hu4785-2(KCTC 10365BP). The humanized monoclonal polypeptide specific for human 4-1BB molecule comprising the amino acid sequence set forth in SEQ ID NO:40 is produced from a cell line Hu4785-2H(KCTC 10440BP). The humanized monoclonal polypeptides specific for human 4-1BB molecule comprising the amino acid sequences set forth in SEQ ID NO:42 is produced from a cell line Hu4785-2K(KCTC 10441BP). A plasmid Hu4785-2H/pCANTAB(KCTC 10365BP) for expressing the heavy chain invariant region of the antibody comprises the humanized monoclonal polypeptide Hu4785-2. A plasmid Hu4785-2H/pcDNA(KCTC 10440BP) for expressing the heavy chain invariant region of the antibody comprises the humanized monoclonal polypeptide Hu4785-2. A plasmid Hu4785-2K/pcDNA(KCTC 10441BP) for expressing the heavy chain invariant region of the antibody comprises the humanized monoclonal polypeptide Hu4785-2.