Novel polypeptide having function of 7keto8aminopelargonic acid synthase of plant and method for inducing growth inhibition and lethality by suppressing expression of the polypeptide

• Main Authors: KIM, TAE HUN, PARK, YU SIN, LEE, DONG HUI, MIN, YONG GI, HWANG, IN TAEK, LEE, SEON U, CHOI, JEONG SEOP, KO, JAE HEUNG, KIM, TAE JUN, CHO, GWANG YEON
• Format: Patent
• Language: English; Korean
• Published: 13.01.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: A plant polypeptide having KAPA synthase function and a method for inducing plant growth inhibition and plant death by inhibiting the expression of the same are provided, thereby inhibiting the biotin biosynthesis, so that it can inhibit the plant growth and cannot affect human and animals. CONSTITUTION: A plant polypeptide having KAPA synthase function has the amino acid sequence of SEQ ID NO: 2, wherein the polypeptide is isolated from Arabidopsis thaliana; and the polypeptide contains aminotransferase class I and II domain at the C-terminal. A polynucleotide encoding the plant polypeptide has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector containing the polynucleotide of SEQ ID NO: 1 is provided, wherein the vector is pCKAPA. A transformed E. coli(KCTC 10210BP) produced with the recombinant vector is provided. An antisense polynucleotide to the polynucleotide of SEQ ID NO: 1 is provided. A recombinant vector containing the antisense polynucleotide is provided, wherein the vector is pSEN-K. A transformant(KCTC 10211BP) produced with the recombinant vector is provided. A method for plant growth inhibition and plant death comprises inhibiting the expression of polypeptide and inhibiting the biotin biosynthesis, wherein the inhibition of the polypeptide expression is accomplished by antisense polynucleotide insertion, gene deletion, gene insertion, T-DNA insertion, homologous recombination or transposon tagging. 본 발명은 식물의 바이오틴 생합성 과정에 관여하는 신규 폴리펩티드 및 이를 암호화하는 폴리뉴클레오티드, 그리고 상기 폴리펩티드의 발현 또는 기능을 억제하여 바이오틴 생합성을 저해함으로써 식물의 생장 억제를 유발하는 방법에 관한 것이다. 바이오틴 생합성 과정은 식물의 생장에는 필수적이나, 동물 및 인간에게는 존재하지 않는다. 따라서, 식물의 바이오틴 생합성 과정에 관여하는 본 발명의 신규 폴리펩티드의 발현 또는 기능을 억제하는 물질은 동물 및 인간에게는 무해한 새로운 제초제로 이용될 수 있다.