Polysaccaride from the citrus peel having gut immunity activity the process for preparation thereof and a use thereof

• Main Authors: YOO, GWANG WON, CHO, HONG YEON, JUN, U JIN, SHIN, DONG HUN, HONG, BEOM SIK, YANG, HAN CHEOL
• Format: Patent
• Language: English; Korean
• Published: 08.04.2003
• Edition: 7
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

PURPOSE: Provided is a composition which activates gut immunity and contains, as an active ingredient, high molecular weight of polysaccharides separated from a water extract of orange peel. The high molecular weight of polysaccharides have anticancer effect and activates gut immunity. Therefore it is effectively used as diet and functional foods or pharmaceutical compositions. CONSTITUTION: A water extract of orange peel is obtained in methanol under refluxing to produce a insoluble fraction. The insoluble fraction is dissolved in water and precipitated in ethanol to produce crude polysaccharides. Thereafter, the crude polysaccharide are ultra filtered to produce polysaccharides having a molecular weight of 100,000Da or more. The polysaccharides are subjected to an anion exchange resin column with a 0.2mol sodium chloride solution and then obtained active fractions are subjected to gel filtration chromatography having a separation capacity of 10,000 to 1,000,000. 본 발명은 귤피로부터 분리한 장관면역 활성화능이 있는 다당류, 그 제조방법 및 그 용도에 관한 것으로, 귤피에 물을 가하여 추출한 다음 메탄올 환류추출하여 비가용 부분만을 수득한 뒤 에탄올 침전시킨 후 한외여과하여 분자량 10만 Da 이상의 고분자 다당류를 분리하고, 상기 다당류를 음이온 교환수지 크로마토그래피, 겔 여과 크로마토그래피를 하여 제조한 고분자 다당류는 장관면역 활성화능이 높아 면역증강제로써 뛰어난 효과가 있을 뿐만 아니라 항암활성을 가지고 있어, 암환자나 면역저하 환자용의 기능성 식품 및 약학적 조성물로 유용하게 사용할 수 있다.