1 Active fractions having inhibitory effects on protein tyrosine phosphatase 1B isolated from Siegesbeckia spp

• Main Authors: AHN, JONG SEOK, BAE, EUN YEONG, KANG, DAE UK, KIM, BO YEON, OH, WON GEUN, AHN, SUN CHEOL, LEE, MYEONG SEON, KIM, MYEONG SU, LEE, CHEOL HO, OH, GU TAEK
• Format: Patent
• Language: English; Korean
• Published: 25.02.2003
• Edition: 7
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

PURPOSE: Provided is an active fraction containing a Siegesbeckia spp. extract which exhibits an excellent effect on inhibition of protein tyrosine phosphatase 1B and reduction of blood sugar levels in Lepr ¬db/Lepr ¬db mouse as a model animal causing type II diabetes and obesity by abnormality of a leptin receptor. It is effective in prevention and treatment of diabetes. CONSTITUTION: Siegesbeckia spp. is extracted in an aqueous alcohol solution and suspended in distilled water. An inactive fraction is removed therefrom with hexane and the aqueous solution, whose inactive fraction is removed, is extracted with chloroform. Thereafter, the concentrate is loaded on a silica gel to dissolve an active matter with a hexane/ethyl acetate solvent, followed by Sephadex LH-20 to dissolve an inactive matter with a chloroform/hexane solvent. And then an active material is extracted, freeze-dried and purified. 본 발명은 희첨(Hui Chum,spp.)으로부터 얻은 단백질 타이로신 탈인산화 효소 1B(protein tyrosine phosphatase 1B, PTP1B) 저해용 활성분획물에 관한 것으로, 더욱 상세하게는 희첨으로부터 단백질 타이로신 탈인산화 효소 1B를 저해하여 인슐린 작용을 강화하여 당뇨병의 주요 증상인 고혈당에 대한 혈당강하 효과를 갖는 활성분획물, 이를 효율적으로 분리, 정제하는 방법 및 이 활성분획물을 유효성분으로 함유하는 당뇨병 예방과 치료, 혈당강하용 생약제에 관한 것이다.