PRIMER SET SPECIFIC TO GENUS STREPTOMYCES rpoB GENE SEQUNCE AND IDENTIFICATION METHOD OF GENUS STREPTOMYCES BY USING THE SAME

• Main Authors: KIM, BUM JOON, KOOK, YOON HOH, KIM, JEONG MI, CHUN, JONG SIK, BAE, GYEONG SUK, LEE, GEUN HWA
• Format: Patent
• Language: English; Korean
• Published: 20.02.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PURPOSE: A primer set specific to genus Streptomyces, rpoB gene fractions, and an identification method of genus Streptomyces by using the same are provided, thereby identifying the genus Streptomyces easily and cheaply. CONSTITUTION: The primer set specific to genus Streptomyces is selected from the group consisting of primers having the nucleotide sequences of SEQ ID NOs: 1 and 2, specifically amplifying RNA polymerase B(rpoB) subunit gene. The polynucleotide is selected from the nucleotide sequences of SEQ ID NOs: 3 to 164 and comprises 306 bp of the rpoB gene fraction of the genus Streptomyces. The identification method of genus Streptomyces comprises the steps of: (i) amplifying the rpoB gene fraction of a strain using the primer set specific to the genus Streptomyces; (ii) sequencing the amplified ropB gene fractions; and (iii) comparing the nucleotide sequences analyzed in the step (ii) and the rpoB gene sequence. 본 발명은 스트렙토미세스속 균주의 rpoB 유전자에 특이적인 PCR 프라이머, 스트렙토미세스속 균주의 동정에 이용될 수 있는 RNA 중합효소 유전자 ()의 306-bp 분절을 나타내는 폴리뉴클레오타이드, 및 이들을 이용한 스트렙토미세스속 균주를 동정하는 방법에 관한 것이다. 본 발명의 동정법은 생장속도가 느리고 다양한 균종이 존재한다는 문제점, 그리고 물질위주 동정 및 16s rDNA 동정이 갖는 문제점을 해결하여, 간편하고, 경제적이고 정확성이 높은 동정방법을 제공한다는 장점이 있어, 향후 스트렙토미세스속 균주 동정에 널리 이용될 수 있다.