NOVEL EXOCHITINASE GENE FROM CELLULOMONAS SP. G13 STRAIN

• Main Authors: JUN, YEONG JUNG, JUNG, GYEONG HWA, CHOI, YEON JIN, SHIN, YONG CHEOL
• Format: Patent
• Language: English; Korean
• Published: 04.10.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE: Provided are a novel exochitinase gene derived from a Cellulomonas sp. G13 strain, the amino acid sequence of a protein encoded thereby, a method for manufacturing a recombinant exochitinase by cloning the same gene, and a method for manufacturing a chitin monomer of NAG using the manufactured exochitinase. CONSTITUTION: The nucleotide sequence and the amino acid sequence of the exochitinase gene are represented by SEQ ID NO:1 and SEQ ID NO:2 respectively. A recombinant exochitinase is manufactured by culturing a transformant transformed with an expression vector including the exochitinase gene. The recombinant exochitinase is used for the manufacture of N-acetyl glucosamine from chitin, disaccharide of NAG, trisaccharide of NAG and oligosaccharide at 55 deg.C and pH 6.0.