NOVEL EXOCHITINASE GENE FROM CELLULOMONAS SP. G13 STRAIN
PURPOSE: Provided are a novel exochitinase gene derived from a Cellulomonas sp. G13 strain, the amino acid sequence of a protein encoded thereby, a method for manufacturing a recombinant exochitinase by cloning the same gene, and a method for manufacturing a chitin monomer of NAG using the manufactu...
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Main Authors | , , , |
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Format | Patent |
Language | English Korean |
Published |
04.10.2002
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | PURPOSE: Provided are a novel exochitinase gene derived from a Cellulomonas sp. G13 strain, the amino acid sequence of a protein encoded thereby, a method for manufacturing a recombinant exochitinase by cloning the same gene, and a method for manufacturing a chitin monomer of NAG using the manufactured exochitinase. CONSTITUTION: The nucleotide sequence and the amino acid sequence of the exochitinase gene are represented by SEQ ID NO:1 and SEQ ID NO:2 respectively. A recombinant exochitinase is manufactured by culturing a transformant transformed with an expression vector including the exochitinase gene. The recombinant exochitinase is used for the manufacture of N-acetyl glucosamine from chitin, disaccharide of NAG, trisaccharide of NAG and oligosaccharide at 55 deg.C and pH 6.0. |
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Bibliography: | Application Number: KR20010015380 |