NOVEL EXOCHITINASE GENE FROM CELLULOMONAS SP. G13 STRAIN

PURPOSE: Provided are a novel exochitinase gene derived from a Cellulomonas sp. G13 strain, the amino acid sequence of a protein encoded thereby, a method for manufacturing a recombinant exochitinase by cloning the same gene, and a method for manufacturing a chitin monomer of NAG using the manufactu...

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Bibliographic Details
Main Authors JUN, YEONG JUNG, JUNG, GYEONG HWA, CHOI, YEON JIN, SHIN, YONG CHEOL
Format Patent
LanguageEnglish
Korean
Published 04.10.2002
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE: Provided are a novel exochitinase gene derived from a Cellulomonas sp. G13 strain, the amino acid sequence of a protein encoded thereby, a method for manufacturing a recombinant exochitinase by cloning the same gene, and a method for manufacturing a chitin monomer of NAG using the manufactured exochitinase. CONSTITUTION: The nucleotide sequence and the amino acid sequence of the exochitinase gene are represented by SEQ ID NO:1 and SEQ ID NO:2 respectively. A recombinant exochitinase is manufactured by culturing a transformant transformed with an expression vector including the exochitinase gene. The recombinant exochitinase is used for the manufacture of N-acetyl glucosamine from chitin, disaccharide of NAG, trisaccharide of NAG and oligosaccharide at 55 deg.C and pH 6.0.
Bibliography:Application Number: KR20010015380