DIFFUSION GRADIENT BIOREACTOR AND EXTRACORPOREAL LIVER DEVICE

• Main Authors: NAUGHTON, BRIAN, A, SIBANDA, BENSON, HALBERSTADT, CRAIG, R
• Format: Patent
• Language: English; Korean
• Published: 25.01.2000
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BANDAGES, DRESSINGS OR ABSORBENT PADS; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROMTHE BODY; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF,TUBULAR STRUCTURES OF THE BODY, E.G. STENTS; ENZYMOLOGY; FILTERS IMPLANTABLE INTO BLOOD VESSELS; FIRST-AID KITS; FISHING; FOMENTATION; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PROSTHESES; SPIRITS; TRAPPING; TREATMENT OR PROTECTION OF EYES OR EARS; VINEGAR; WINE

PURPOSE: A bioreactor is provided to grow and to maintain a three-dimensional tissue culturing material being composed of multi-functional cells being operated through a diffusion and an osmotic pressure in one or both sides of the cultivated tissue. CONSTITUTION: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extra corporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media, allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood. 3차원 조직을 생장시키기 위한 조직 처리 바이오리엑터에 대해 상술한다. 세포는 메쉬에 접종시키고, 두 가지 배지 흐름을 제공하는데 각각은 세포의 다른 측면에 접하도록 한다. 배지 흐름에는 다른 농도의 영양분을 포함하여 확산 차에 의해 영양분이 세포로 운반된다. 바이오리엑터는 간 조직을 생장시키기 위해 이용될 수 있고 체외 간 보조 장치로 이용될 수 있는데 이때 혈액은 3차원 조직에 노출된다. 환자의 혈액 또는 혈장은 간 조직으로 흐르게 된다. 간 조직은 또한 영양분 및 기체를 공급하는 배지에 반대측면에서 노출된다. 장치에는 혈액, 혈장, 배지사이에 다공성 경계면을 제공하여 확산 차이에 의해 영양분 및 단백질이 운반되어 환자의 혈액을 투석한다.